ABSTRACT
The goal of this study was to determine the characteristics of the horizontal cervical wrinkle and to investigate histological feature, especially with respect to elastin and collagen fiber. Histologic sample were harvested from two fresh adult cadavers where the cervical wrinkle is in the neck. The tissue sections were stained with hematoxylin and eosin (HE) or Masson's Trichrome. In sections of neck skin, keratinization was observed in the epidermis, and many collagen fibers were observed in the dermis layer as in other skin. Specifically, a lot of short and curly elastic fiber were observed between the collagen fibers in the dermis. These elastic fibers were not stained with eosin and observed in gray. This long ligament-like structures were observed in the dermis. The ligament-like structures were stained with dark red by trichrome. These results indicate that these ligament-like structures are neither typical ligament nor typical smooth muscles. The results obtained from present study showed a ligamentous structure originating from the fascial layer (platysma muscle or investing layer). The results may help to understand the reasons about the formation of horizontal cervical wrinkle.